We have begun assigning group membership according to published DNA results, beginning with descendants and female line relatives of Anna of Osterreich (Anna of Habsburg) who lived 1280 to 1327. We can be reasonably certain about her Hg H 16519C haplotype on account of several peer-review published studies:

a) Mitochondrial DNA analysis on remains of a putative son of Louis XVI, King of France and Marie-Antoinette, (1998), European Journal of Human Genetics, (6), 383-395.

b) Mitochondrial DNA analysis of the putative heart of Louis XVI, son of Louis XVI and Marie-Antoinette; (2001), Jehaes, Els; Pfieffer, Heidi, Toprak; Decorte, Ronny; Brinkmann, Bernd; and Cassiman, Jean-Jacques. European Journal of Human Genetics, (9), 185-190.

c) Genetic genealogy reveals true Y haplogroup of House of Bourbon contradicting recent identification of the presumed remains of two French Kings, (2014), European Journal of Human Genetics, (22) 681-687.

C counters the conclusions reached in an earlier paper by producing a pair of genealogical trees that were constructed using the Y and mtDNA results obtained from living persons with established genealogical pedigrees. The derived mtDNA chart shows that descendants from the ancestress-in-common, Anna of Osterreich (Anna of Habsburg), show female-line descent in these and other royal houses: Habsburg, d'Albret, Navarre, Savoy, Bourbon, Burgundy, Bavaria (Wittelsbach), Brieg, Glowgow, Ballenstadt, Brandenburg, Henneberg, Hohenzollern, Thuringia, Saxony, Zagan, Mansfield, Stolberg, Querfurt, Reuss, Schonburg, Sohms-Laubach, Salm-Kryburg, Wurttemburg, Oettingen-Oettingen, Brunswich-Wulfenbuttel, and all female line descendants of Marie-Theresa, Archduchess of Austria including Queen Marie-Antoinette and Queen Anna of Romania..  

Because the published mitochondrial DNA results were given in the revised Cambridge Reference Sequence (rCRS), membership in this group is also determined by an individual's rCRS HV 1 haplotype. The rCRS is most appropriate for persons in the H haplogroup, as the rCRS sample was drawn from a person of the H haplogroup.