Open to any individuals with the surname of Pullen or the most common Soudex variations. It does not matter if you think that you are or are not related. The more individuals who do this test the more reliable the DNA test will end up being. If you are a male you can use either/both the Mtdna and if you are female use the Mtdna. The Y-DNA is specifically used for any male with the surname of Pullen or any Soundex variation. The Mtdna goes further back than the Y-DNA but remember it will cross over many females because of the name change due to marriage.